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ELISA is a simple and highly sensitive method of analysis that allows for simultanous and rapid quantification of a large number of samples.
The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labelled antibody or antigen. Uppon addition of a non-colored reagent the enzyme produces a color reaction where the color intensity is directly or inversely propotional to the concentration of the analyte in the sample.