MAP3K4/MEK kinase-4 Antibody, ALEXA FLUOR 488
Basic information
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Name
MAP3K4/MEK kinase-4 Antibody, ALEXA FLUOR 488
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Price
380 EUR
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Size
100ul
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Catalog number
bs-11883R-A488
Detailed information
Long name
MAP3K4/MEK kinase-4 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known as
Anti-MAP3K4/MEK kinase-4 PAb ALEXA FLUOR 488
Category
Conjugated Primary Antibodies
Conjugated with
ALEXA FLUOR® 488
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
MAP3K4/MEK kinase-4
Specificity
This is a highly specific antibody against MAP3K4/MEK kinase-4.
Modification
Unmodified
Modification Site
None
Clonality
Polyclonal
Clone
Polyclonal antibody
Concentration
1ug per 1ul
Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MAP3K4/MEK kinase-4
Tested applications
IF(IHC-P)
Recommended dilutions
IF(IHC-P)(1:50-200)
Crossreactivity
Human, Mouse, Rat
Cross-reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen
Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK path-way. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.
Purification
Purified by Protein A.
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission
499nm/519nm
Synonyms
FLJ42439; KIAA0213; M3K4_HUMAN; MAP three kinase 1; MAP3K4; MAPK/ERK kinase kinase 4; MAPKKK4 antibodyMEK kinase 4; MEKK 4; MEKK4; Mitogen-activated protein kinase kinase kinase 4; MTK1; PRO0412; RP3-473J16.4
Properties
For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-MAP3K4/MEK kinase-4 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Conjugation
Alexa Fluor
French translation
anticorps