Recombinant Human MAP4K5 / MEKKK5 Protein (His & GST tag) 50µg
Basic information
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Name
Recombinant Human MAP4K5 / MEKKK5 Protein (His & GST tag) 50µg
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Price
553 EUR
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Size
50µg
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Catalog number
PKSH030349-50µg
Detailed information
Synonym
GCKR;KHS;KHS1;MAPKKKK5
Activity
NA
Sequence
Met 1-Tyr 846
Fusion tag
N-His & GST
Accession
NP_006566.2
Expressed Host
Baculovirus-Insect Cells
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Purity
＞82 % as determined by SDS-PAGE
Endotoxin
＜1.0 EU per µg as determined by the LAL method
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Mol Mass
123 kDa
AP Mol Mass
116 kDa
Formulation
Supplied as sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM TCEP, pH 8.5
Reconstitution
Please refer to it for detailed information.
Background
Mitogen-activated protein kinase kinase kinase kinase 5, also known as Kinase homologous to SPS1/STE20, MAPK/ERK kinase kinase kinase 5, MEK kinase kinase 5 and MAP4K5, is a cytoplasm protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. MAP4K5 is ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate. It contains one CNH domain and one protein kinase domain. MAP4K5 is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. MAP4K5 has been shown to interact with CRKL and TRAF2. This kinase was shown to activate Jun kinase in mammalian cells. MAP4K5 is an early component of MAP kinase signal cascades. It may play a role in the response to environmental stress. MAP4K5 appears to act upstream of the JUN N-terminal pathway.
Conjugation
histidine
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source
Recombinants or rec. proteins
Group
recombinants