Need help?

+390236006593 Hours: 8am-7pm M-Fri

Basic information

  • Name

    Recombinant Human MKK6 / MEK6 / MAP2K6 Protein (207 Ser/Asp, 211 Thr/Asp, His & GST tag)

  • Price

    445 EUR

  • Size

    20µg

  • Catalog number

    PKSH030414-20µg

Detailed information

Synonym

MAPKK6;MEK6;MKK6;PRKMK6;SAPKK-3;SAPKK3

Activity

The specific activity was determined to be 1250 nmol/min/mg using inactive MAPK14 as substrate.

Sequence

Met 1-Asp 334, Ser 207/Asp, Thr 211/Asp

Fusion tag

N-His & GST

Accession

P52564-1

Expressed Host

Baculovirus-Insect Cells

Shipping

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.

Purity

>88 % as determined by SDS-PAGE

Endotoxin

<1.0 EU per µg as determined by the LAL method

Stability and Storage

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Mol Mass

65.3 kDa

AP Mol Mass

60 kDa

Formulation

Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol

Background

Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6 and MKK6, is a protein which belongs to the?protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one?protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.

Conjugation

histidine

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Source

Recombinants or rec. proteins

Group

recombinants