Recombinant Human MKK6 / MEK6 / MAP2K6 Protein (207 Ser/Asp, 211 Thr/Asp, His & GST tag)
Basic information
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Name
Recombinant Human MKK6 / MEK6 / MAP2K6 Protein (207 Ser/Asp, 211 Thr/Asp, His & GST tag)
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Price
445 EUR
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Size
20µg
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Catalog number
PKSH030414-20µg
Detailed information
Synonym
MAPKK6;MEK6;MKK6;PRKMK6;SAPKK-3;SAPKK3
Activity
The specific activity was determined to be 1250 nmol/min/mg using inactive MAPK14 as substrate.
Sequence
Met 1-Asp 334, Ser 207/Asp, Thr 211/Asp
Fusion tag
N-His & GST
Accession
P52564-1
Expressed Host
Baculovirus-Insect Cells
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Purity
＞88 % as determined by SDS-PAGE
Endotoxin
＜1.0 EU per µg as determined by the LAL method
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Mol Mass
65.3 kDa
AP Mol Mass
60 kDa
Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol
Background
Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6 and MKK6, is a protein which belongs to the?protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one?protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Conjugation
histidine
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source
Recombinants or rec. proteins
Group
recombinants