Rat ERK1/2(Extracellular Signal Regulated Kinase 1/2) EIA Kit
Basic information
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Name
Rat ERK1/2(Extracellular Signal Regulated Kinase 1/2) EIA Kit
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Price
492 EUR
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Size
96Test
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Catalog number
E-EL-R2529-96T
Detailed information
Detection Type
Colormetric
Target Species
Rat
Target Synonym
NA
Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat ERK1/2. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ERK1/2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ERK1/2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ERK1/2. You can calculate the concentration of Rat ERK1/2 in the samples by comparing the OD of the samples to the standard curve.
Sensitivity
0.47ng/mL
Detection Range
0.78~50ng/mL
Target Name
ERK1/2
UNIProt ID
P21708
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Rat ERK1/2(Extracellular Signal Regulated Kinase 1/2) EIA Kit
Type
Sandwich
Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus