MAPKAP Kinase 2 Antibody
Basic information
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Name
MAPKAP Kinase 2 Antibody
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Price
263 EUR
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Size
0.1ml
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Catalog number
bs-2908R
Detailed information
Long name
MAPKAP Kinase 2 Primary Polyclonal Antibody
Also known as
MAPKAP Kinase 2 PAb
Category
Primary Antibodies
Conjugation
Unconjugated
Target Antigen
MAPKAP Kinase 2
Specificity
This is a highly specific antibody against MAPKAP Kinase 2.
Modification(s)
None
Modification site(s)
Unmodified antibody
Clonality
Polyclonal antibody
Clone number
Polyclonal antibody
Concentration
1ug per 1ul
Immunogen range
337-363/400
Subcellular location
Cytoplasm, Nucleus
Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MAPKAP Kinase 2
Gene ID number
9261
Swiss Prot
P49137
Tested Applications
WB, IHC-P, IF(IHC-P)
Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Cross reactivity
Human, Mouse, Rat, Bovine
Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the target antigen
Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA, HSF1, HSP27/HSPB1, KRT18, KRT2, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA, PABPC1 and TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA and PARN, leading to stabilize GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
Purification method
This antibody was purified via Protein A.
Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
Synonym names
MK2; MK-2; MAPKAP-K2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAPK-2; MAPKAPK2
Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps