Human ERK1 (Extracellular Signal Regulated Kinase 1) ELISA Kit
Basic information
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Name
Human ERK1 (Extracellular Signal Regulated Kinase 1) ELISA Kit
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Price
759 EUR
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Size
96 Tests
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Catalog number
EKE61163
Detailed information
Estimated Turnaround Time
6-11 business days
Target Name
NA
Target's alterntive name
ERK1
Species Reactivity
Human
Sample Type
Serum, Plasma, Biological Fluids
Detection Range
0.31-20 ng/mL
Sensitivity
0.19 ng/mL
Precision
NA
Detection Method
Colorimetric
Assay Time
4.5 hours
Application
For research use only. Not for diagnostic procedures.
Assay Type
Sandwich
Shipping Conditions
Ice packs
Storage Temperature
Short term: 4°C; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life
Use Human ERK1 (Extracellular Signal Regulated Kinase 1) ELISA Kit before 6 months
Specificity
NA
Cross Activity
No significant cross-reactivity or interference was observed.
Test Principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ERK1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ERK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ERK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ERK1. The concentration of Human ERK1 in samples can be calculated by comparing the OD of the samples to the standard curve.
Stability
NA
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Research Area
Cancer, Neuroscience, Signal transduction, Stem cells
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED