Rat MAP2K1(Mitogen Activated Protein Kinase Kinase 1) ELISA Kit
Basic information
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Name
Rat MAP2K1(Mitogen Activated Protein Kinase Kinase 1) ELISA Kit
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Price
608 EUR
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Size
96T
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Catalog number
ELK7539
Detailed information
Alternative Names
MAPKK1; MEK1; MKK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1
Assay Type
Sandwich
Sensitivity
0.063ng/mL
Standard
10ng/mL
Detection range
0.156-10ng/mL
Assay length
3h
Research Area
Signal transduction;
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase Kinase 1 (MAP2K1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus