anti-MAPK1 Antibody [4C11C11C4]
ERK2, a MAPK type protein kinase, is a member of the Ras MAPK pathway. This pathway modulates the activity of transcription factors, structural proteins and other protein kinases, leading to biological responses such as cell proliferation and differentiation. Inactive ERK2 associates with MAPK/ERK kinases (MEK) 1 and 2 in the cytosol. Upon stimulation, MEK1/2 phosphorylates and activates ERK2 causing dissociation of the MEK/ERK complex. A portion of the activated ERK2 pool forms dimers and translocates to the nucleus to activate nuclear proteins, such as ELK-1 and MSK1. Cytosolic ERK 2 acts on a different set of substrates, including MNK1/2 and MAPKAP-K1.
MAPK1, ERK, ERK-2, ERK2, MAP kinase 1, MAPK 2, MAPK2, MAP kinase isoform p42, MAPK 1, p41mapk, PRKM2, MAP kinase 2, p41, p42-MAPK, PRKM1, ERT1, p38, p40, p42MAPK, Protein tyrosine kinase ERK2
Ab raised in
MAPK1 monoclonal antibody was made against Ni-NTA purified full length recombinant ERK2 expressed in E. coli strain BL21 (DE3).
ELISA, Western Blot, immuno-histo-chemistry
MAPK1 antibody can be used in Western Blot starting at 1:500 - 1:3000, immunohistochemistry starting at 1:400, immunoprecipitation, and immunofluorescence.
Suspention, pH, azide
PBS, 0.01% sodium azide.
Store MAPK1 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.
Vial with antibody
NCBI GENE nr
mitogen-activated protein kinase 1
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.