Mitogen-activated protein kinase kinase kinase (MEKK1) is a serine-threonine kinase that regulates sequential protein kinase pathways, generally activated by stress and by various mitogenic stimuli or growth factors. Targeted disruption the MEKK1 in mouse embryonic stem cells showed lost or altered responses of the c-jun aminoterminal kinase (JNK) to microtubule disruption and cold stress but activated JNK normally in response to heat shock, anisomycin, and ultraviolet irradiation. Activation of JNK was lost and that of extracellular signal-regulated protein kinase (ERK) was diminished in response to hyperosmolarity and serum factors in MEKK1-/-cells. Loss of MEKK1 expression resulted in a greater apoptotic response of cells to hyperosmolarity and microtubule disruption. When activated by specific stresses that alter cell shape and the cytoskeleton, MEKK1 signals to protect cells from apoptosis. Suitable for use in ELISA, Western Blotting. Other applications not tested. Reacts specifically with MEKK1 protein detecting a monomer species migrating at ~69kD and a trimer species migrating at ~200kD. Cross-reactivity with other other proteins has not been observed.
MAP3K1, MAPK/ERK kinase kinase 1, MAPKKK1, MAP/ERK kinase kinase 1, MEK kinase 1, MEKK, MEKK 1, MEKK1, SRXY6
Ab raised in
MAP3K1 antibody was made against amino acids 130 - 300 of MAP3K1 (Human).
ELISA, Western Blot, immuno-histo-chemistry
MAP3K1 antibody can be used in immunohistochemistry starting at 1.25 - 2.5 ug/mL.
Suspention, pH, azide
PBS, pH 7.4, 0.1% sodium azide.
Store MAP3K1 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.
Vial with antibody
NCBI GENE nr
mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.