MAPK8 Polyclonal Antibody
Basic information
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Name
MAPK8 Polyclonal Antibody
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Price
245 EUR
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Size
50 μg
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Catalog number
CAC07051
Detailed information
Other name
Mitogen-activated protein kinase 8,JNK-46,Stress-activated protein kinase 1c,SAPK1c,Stress-activated protein kinase JNK1,c-Jun N-terminal kinase 1,MAPK8,JNK1,PRKM8,SAPK1,SAPK1C
Immunogen
Recombinant human Mitogen-activated protein kinase 8 protein (5-300AA)
Verified reactivity
Human, Mouse
Verified applications
ELISA,WB,IHC,IF. Recommended dilution:WB:1:1000-1:5000,IHC:1:20-1:200,IF:1:50-1:200
Raised in
Rabbit
Clonality
Polyclonal
Protein number
P45983
Gene number
Please refer to GenBank
NCBI number
Please refer to NCBI
Purity
Protein G purified, >95%
Storage recommendation
Aliquot and store at -20°C. Minimize freezing and thawing.
Use before
1 year
Shipping requirements
Blue ice
Estimated production time
3-7 business days
Supplementary information
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).
Notes
For research use only. Not for diagnostic procedures.
Properties
If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation
anticorps