Rabbit Anti-Human MAP3K12 (pSer269) Polyclonal: Biotin
Basic information
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Name
Rabbit Anti-Human MAP3K12 (pSer269) Polyclonal: Biotin
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Price
393 EUR
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Size
100 µl
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Catalog number
SPC-955D-BI
Detailed information
Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser269 of human MAP3K12 (AA266-272)
Antibody's target
Human MAP3K12 (pSer269)
Antibody's full description
Rabbit Anti-Human MAP3K12 (pSer269) Polyclonal
Antibody's category
Polyclonal Antibodies
Antibody's other name
Dlk Antibody, Dual leucine zipper bearing kinase Antibody, Leucine-zipper protein kinase Antibody, M3K12_HUMAN Antibody, MAPK-upstream kinase Antibody, MEKK12 Antibody, Mitogen-activated protein kinase kinase kinase 12 Antibody, MUK Antibody, ZPK Antibody, ZPKP1 Antibody
Verified applications
WB, AM
Raised in
Rabbit
Antibody's reactivity
Human
Antibody's recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from
Peptide Affinity Purified
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration
See included datasheet or contact our support service
Antibody's specificity
Detects 52.536 kDa.
Storage recommendations
-20°C
Shipping recommendations
Blue Ice or 4°C
Certificate of analysis
A 1:250 dilution of SPC-955 was sufficient for detection of MAP3K12 (pSer269) in 10 µg of human umbilical vein cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell
Cytoplasm, Membrane
Tissue specificity
See included datasheet or contact our support service.
Scientific context
MAP3K12 is an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
Bibliography
Refer to PubMed
Released date
29/Aug/2016
NCBI number
NP_006292
Gene number
7786
Protein number
Q12852
PubMed number
Refer to PubMed
Tested applications
To be tested
Tested reactivity
To be tested
Antibody's datasheet
Contact our support service to receive datasheet or other technical documentation.
Representative figure link
No Data Available
Warning information
Non-hazardous
Country of production
Canada
Total weight (kg)
1.4
Net weight (g)
0.1
Stock availabilit
In Stock
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation
Biotinylated
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name
Oryctolagus cuniculus