Rabbit Anti-Human MAP4K4 (pThr187) Polyclonal: ATTO 488
Basic information
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Name
Rabbit Anti-Human MAP4K4 (pThr187) Polyclonal: ATTO 488
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Price
397 EUR
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Size
100 µl
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Catalog number
SPC-994D-A488
Detailed information
Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr187 of human MAP4K4 (AA184-190)
Antibody's target
Human MAP4K4 (pThr187)
Antibody's full description
Rabbit Anti-Human MAP4K4 (pThr187) Polyclonal
Antibody's category
Polyclonal Antibodies
Antibody's other name
Hepatocyte progenitor kinase like/germinal center kinase like kinase Antibody, HGK Antibody, HPK/GCK like kinase Antibody, KIAA0687 Antibody, M4K4_HUMAN Antibody, MAP4K4 Antibody, MAPK/ERK kinase kinase kinase 4 Antibody, MEK kinase kinase 4 Antibody, MEKKK 4 Antibody, Mitogen activated protein kinase kinase kinase kinase 4 Antibody, Nck interacting kinase Antibody, NIK Antibody
Verified applications
WB, AM
Raised in
Rabbit
Antibody's reactivity
Human
Antibody's recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from
Peptide Affinity Purified
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration
See included datasheet or contact our support service
Antibody's specificity
Detects 142.101 kDa.
Storage recommendations
-20°C
Shipping recommendations
Blue Ice or 4°C
Certificate of analysis
A 1:250 dilution of SPC-994 was sufficient for detection of MAP4K4 (pThr187) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell
Cytoplasm
Tissue specificity
Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Scientific context
MAP4K4 appears to act upstream of the JUN N-terminal pathway. It phosphorylates SMAD1 on Thr-322.
Bibliography
Refer to PubMed
Released date
29/Aug/2016
NCBI number
NP_001155032
Gene number
9448
Protein number
O95819
PubMed number
Refer to PubMed
Tested applications
To be tested
Tested reactivity
To be tested
Antibody's datasheet
Contact our support service to receive datasheet or other technical documentation.
Representative figure link
No Data Available
Warning information
Non-hazardous
Country of production
Canada
Total weight (kg)
1.4
Net weight (g)
0.1
Stock availabilit
In Stock
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name
Oryctolagus cuniculus