Rabbit Anti-Human ZAK (pThr161+pThr162) Polyclonal: Biotin
Basic information
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Name
Rabbit Anti-Human ZAK (pThr161+pThr162) Polyclonal: Biotin
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Price
393 EUR
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Size
100 µl
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Catalog number
SPC-1029D-BI
Detailed information
Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr161 and Thr162 of human MLTK (AA158-165)
Antibody's target
Human ZAK (pThr161+pThr162)
Antibody's full description
Rabbit Anti-Human ZAK (pThr161+pThr162) Polyclonal
Antibody's category
Polyclonal Antibodies
Antibody's other name
AZK antibody, Cervical cancer suppressor gene 4 protein antibody, HCCS 4 antibody, HCCS-4 antibody, Human cervical cancer suppressor gene 4 protein antibody, Leucine zipper and sterile alpha motif containing kinase antibody, Mitogen activated protein kinase kinase kinase MLT antibody, Mixed lineage kinase related kinase antibody, MLK 7 antibody, MLK related kinase antibody, MLK-like mitogen-activated protein triple kinase antibody, MLK-related kinase antibody, MLK7 antibody, Mlklak antibody, MLT antibody, MLTK_HUMAN antibody, ZAK antibody
Verified applications
WB, AM
Raised in
Rabbit
Antibody's reactivity
Human
Antibody's recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from
Peptide Affinity Purified
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration
See included datasheet or contact our support service
Antibody's specificity
Detects 91.181 kDa.
Storage recommendations
-20°C
Shipping recommendations
Blue Ice or 4°C
Certificate of analysis
A 1:250 dilution of SPC-1029 was sufficient for detection of ZAK (pThr161+pThr162) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell
Cytoplasm, Nucleus
Tissue specificity
Ubiquitously expressed
Scientific context
Stress activated and proapoptotic member of the MAPKKK family. Regulates the JNK and p38 pathways. Involved in regulation of S and G2 cell cycle checkpoints by direct phosphorylation of CHEK2. MLTK causes cell shrinkage and disruption of actin stress fibers and phosphorylates histone H3 (Ser-28). It was suggested to play a role in cell transformation and oncogenesis.
Bibliography
Refer to PubMed
Released date
29/Aug/2016
NCBI number
NP_057737
Gene number
51776
Protein number
Q9NYL2
PubMed number
Refer to PubMed
Tested applications
To be tested
Tested reactivity
To be tested
Antibody's datasheet
Contact our support service to receive datasheet or other technical documentation.
Representative figure link
No Data Available
Warning information
Non-hazardous
Country of production
Canada
Total weight (kg)
1.4
Net weight (g)
0.1
Stock availabilit
In Stock
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation
Biotinylated
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name
Oryctolagus cuniculus