JNK Activity Assay Kit, KinaseSTAR
Basic information
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Name
JNK Activity Assay Kit, KinaseSTAR
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Price
642 EUR
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Size
40 assays
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Catalog number
K431-40
Detailed information
Kit's description
c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. The JNK Activity Immunoassay Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. Activity of the JNK is then determined in a kinase reaction using recombinant c-Jun as substrate. Phosphorylation of the c-Jun can be analyzed by Western blot analysis using a phospho-c-Jun specific antibody. The kit specifically detects JNK, other kinase activity would not be detected.
Highlights
• Detection method: Western blot analysis using a phospho:c:Jun specific antibody at 1:1000 dilutions • Species reactivity: Mammalian • Applications: The JNK Activity Immunoassay Kit utilizes a JNK:specific antibody to immunoprecipitate JNK from cell lysate. JNK:specific activity is then analyzed by determining the phosphorylation of c:Jun by Western blotting using a phospho:c:Jun specific antibody.
Kit's benefits
• Simple procedure • The kit is suitable for both research and drug discovery • The kit specifically detects JNK, other kinase activity would not be detected
Category
JNK
Kit's other name
none
Contents
• Kinase Extraction Buffer • JNK Specific Antibody • Protein A Sepharose • c-Jun Protein/ATP Mixture • Kinase Assay Buffer • Phospho-cJun Specific Antibody
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue lysates
Test
Biovision supplies other types of Assays as 1.