MAP Kinase, Activated(Diphosphorylated ERK1&2) Antibody ( MAPK
Basic information
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Name
MAP Kinase, Activated(Diphosphorylated ERK1&2) Antibody ( MAPK
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Price
320 EUR
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Size
0,1 mg
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Catalog number
MA1055
Detailed information
Target antigen
MAP Kinase, ActivatedDiphosphorylated ERK1&2
Clonality
Monoclonal antibody
Clone
MAPK-YT
Raised in
mice
Type of the antibody
IgG1 monoclonal antibody
Product form
freeze-dried
Reacts with species:
Human, mouse, rat, yeast
Analyses
WB,IHC-P,ICC
Immunogen
A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH.
Product configuration
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Purification
Ascites
Solubilization
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
Storage condtions
Keep the MAP Kinase, Activated(Diphosphorylated ERK1&2) Antibody ( MAPKat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
Tips
The MAP Kinase, Activated(Diphosphorylated ERK1&2) Antibody ( MAPK did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
Background
In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein(MAP) kinase pathways. The central core of each MAP kinase(MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase(MAPKKK) phosphorylates and activates a specific MAPK kinase(MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.
Related articles
1. Rodriguez-Viciana, P.; Tetsu, O.; Tidyman, W. E.; Estep, A. L.; Conger, B. A.; Santa Cruz, M.; McCormick, F.; Rauen, K. A. : Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome. Science 311: 1287-1290, 2006. 2. Scholl, F. A.; Dumesic, P. A.; Barragan, D. I.; Harada, K.; Bissonauth, V.; Charron, J.; Khavari, P. A. : Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Dev. Cell 12: 615-629, 2007.
Gene Name
MAPK1; MAPK3
Protein Name
Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3
Gene Full Name
mitogen-activated protein kinase 1; mitogen-activated protein kinase 3
Synonyms
ERK|p38|p40|p41|ERK2|ERT1|MAPK2|PRKM1|PRKM2|P42MAPK|p41mapk|ERK1|ERT2|ERK-1|PRKM3|P44ERK1|P44MAPK|HS44KDAP|HUMKER1A|p44-ERK1|p44-MAPK|Extracellular signal-regulated kinase 2|MAP kinase isoform p42|Mitogen-activated protein kinase 2
Uniprot ID
Human(P28482), Mouse(P63085), Rat(P63086), Human(P27361), Mouse(Q63844), Rat(P21708)
Entrez GeneID
Human(5594), Mouse(26413), Rat(116590), Human(5595), Mouse(26417), Rat(50689)
Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps