MAPK1/3 Antibody
Basic information
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Name
MAPK1/3 Antibody
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Price
320 EUR
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Size
0,1 mg
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Catalog number
PA1049
Detailed information
Target antigen
MAPK1/3
Clonality
Polyclonal antibody
Clone
Polyclonal antibody
Raised in
rabbit
Type of the antibody
IgG polyclonal antibody
Product form
freeze-dried
Reacts with species:
human, rat Predicted to work with: mouse
Analyses
WB,IHC-P
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MAPK1/3(171-184aa ARVADPDHDHTGFL), identical to the related rat and mouse sequences.
Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Purification
Immunogen affinity purified.
Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
Storage condtions
Keep the MAPK1/3 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
Tips
The MAPK1/3 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
Background
MAPK1(ERK2) shares high homology with MAPK3(ERK1). MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Mitogen-activated protein(MAP) kinases [also known as Erks] have been established to function as important mediators of signal transduction by growth factor receptors. ERK1/ERK2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level. ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF at amino acids 117 and 201 within HMG boxes 1 and 2, preventing their interaction with DNA. Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2.
Related articles
1. Bhalla, U. S.; Ram, P. T.; Iyengar, R. : MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Science 297: 1018-1023, 2002. 2. Li, L.; Wysk, M.; Gonzalez, F. A.; Davis, R. J. : Genomic loci of human mitogen-activated protein kinases. Oncogene 9: 647-649, 1994.
Gene Name
MAPK1
Protein Name
Mitogen-activated protein kinase 1(MAP kinase 1/MAPK 1)
Gene Full Name
mitogen-activated protein kinase 1
Synonyms
ERT1|Extracellular signal-regulated kinase 2
Uniprot ID
P28482
Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps