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Basic information

  • Name

    Human MAP Kinase (MAPK) Signaling Pathway qPCR array

  • Price

    187 EUR

  • Size

    1 x 96 well primer plate

  • Catalog number

    ANGNH-MAPK1H1

Detailed information

Genes

BRAF, EGFR, HSPB1, MAP2K2, MAP3K2, MAP3K3, MAP4K1, MAPK10, MOS, MYC, RAF1, TNF, ARAF, HSPA5, MAP2K4, MAP2K6, MAP2K7, MAP3K1, MAPK1, MAPK14, MAPK3, MAPK8, MEF2C, MST1, PAK1, SFN, CCNA1, CCNA2, CCNB1, CCNB2, CCND1, CCND2, CCND3, CCNE1, CDC42, CDK2, CDK4, CDK6, CDKN1A, CDKN1B, CDKN1C, CDKN2A, CDKN2B, CDKN2C, CDKN2D, HRAS, LAMTOR3, MAP2K1, MAPK13, MAPK6, MAPK7, DLK1, RAC1, PRDX6, KSR1, MAPKAPK2, GRB2, MAPKAPK3, NRAS, MAP3K4, MAPK8IP2, MAPK9, ATF2, CHUK, COL1A1, CREB1, CREBBP, E2F1, EGR1, ELK1, ETS1, ETS2, FOS, JUN, MAP2K3, MAP2K5, MAPK11, MAPK12, MAX, NFATC4, RB1, SMAD4, TP53, MKNK1.

Sequence disclosure

We do not standard disclose the sequence of our carefully selected and oprimized RT PCR primers.

Excel template

We will supply you with a microsoft excel template to analyse the qPCR results and see the activation, or supression of genes of interestof your profiler.

Sign Arrays

signal transduction

Custom 96 or 384 well qPCR primers

1 euro or USD per human gene added to the qPCR MAP Kinase (MAPK) Signaling Pathway profiling plate.

Roche LightCycler compatibility

The LightCycler 480, for 96 well qPCR: XXXH1-RXS for 384 well: XXXH2-RXS. With perfect Master Mix Sybr Green: PMSX-WXS, 82 Euro

AB cycler RT PCR blocks

The Applied Biosystems Step One Plus Real-Time System 96 well is used with the qPCR XXXH1-FXS with Perfect Master Mix SYBR Green PMSX-RXS, 82 USD and ABI 7900 HT (standard block) XXXH1-AXS, XXXH2-AXS, PMSX-RXS and ViiA7 system (standard block), XXXH1-AXS, XXXH2-AXS, PMSX-LRXS and QuantStudio 6, 7, 12K Flex System (standard block)

Biorad qPCR cyclers

The RT PCR CFX 96 cycler, CFX 384, CFX Connect, Opticon, Chomo, iQ and MyiQ Cycler are compatible withour Perfect Master Mix SYBR Green. Contact us for the reference.

Pathway profiling

The expression RT PCR profiler array is supplied in 96 and 384 well primer plates. They are used to profile cDNA from human tissues, cells or body fluids. We can perform signaling pathways analysis with only 10-100 cells with the 2018 Direct lysis to SignArray reagent. For rare or precious sampleswe use the SpeAmpn reagents to analyze until 4 signaling pathways with only 5-50ng starting amount of RNA. The adapted hot start SYBR Green qPCR kit apropriate to your Real Time PCR cycler is supplied at 82$ or Euro. Please supply us with your qPCR cyler model when ordering.

Pathway profiling outsourcing

We have a large collection of cell lines and can perfome the MAP Kinase (MAPK) Signaling Pathway expression profiling for you. You can send us your tissues or we have a library of tissues and can perform the RNA extraction, make the cDNA and perform the RT-PCR and qPCR for you.

QPCR Master Mix

We suppy PMS reagent or Perfect Master Mix SYBRG for SignArrays 96 at 10 μl of PMS per reaction for 20 μl final volume. The reference is: PMS1-Z2S, 82 euro

Single gene qPCR

Perfect Master Mix PROBE, PMP1-Z50 for 50 reactions, price: 75 eur/USD

Distribution

Gentaur ensures worldwide distribution of Human MAP Kinase (MAPK) Signaling Pathway qPCR array except for spain

Description

More than 1 can be supplied by AnyGenes.Cell nucleus signaling proteins and molecules are part of a complex system of communication that governs basic cellular activities and coordinates cell actions. The ability of cells to perceive and correctly respond to their microenvironment is the basis of development, tissue repair, and immunity as well as normal tissue homeostasis. Errors in cellular information processing are responsible for diseases such as cancer, autoimmunity, and diabetes. By understanding cell signaling, diseases may be treated effectively and, theoretically, artificial tissues may be created.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.