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Basic information

  • Name

    MAP3K4/MEK kinase-4 Antibody, ALEXA FLUOR 488

  • Price

    380 EUR

  • Size

    100ul

  • Catalog number

    bs-11883R-A488

Detailed information

Long name

MAP3K4/MEK kinase-4 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Also known as

Anti-MAP3K4/MEK kinase-4 PAb ALEXA FLUOR 488

Category

Conjugated Primary Antibodies

Conjugated with

ALEXA FLUOR® 488

Host Organism

Rabbit (Oryctolagus cuniculus)

Target Antigen

MAP3K4/MEK kinase-4

Specificity

This is a highly specific antibody against MAP3K4/MEK kinase-4.

Modification

Unmodified

Modification Site

None

Clonality

Polyclonal

Clone

Polyclonal antibody

Concentration

1ug per 1ul

Source

This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MAP3K4/MEK kinase-4

Tested applications

IF(IHC-P)

Recommended dilutions

IF(IHC-P)(1:50-200)

Crossreactivity

Human, Mouse, Rat

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Background of the antigen

Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK path-way. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.

Purification

Purified by Protein A.

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Excitation emission

499nm/519nm

Synonyms

FLJ42439; KIAA0213; M3K4_HUMAN; MAP three kinase 1; MAP3K4; MAPK/ERK kinase kinase 4; MAPKKK4 antibodyMEK kinase 4; MEKK 4; MEKK4; Mitogen-activated protein kinase kinase kinase 4; MTK1; PRO0412; RP3-473J16.4

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-MAP3K4/MEK kinase-4 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Conjugation

Alexa Fluor

French translation

anticorps