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Basic information

  • Name

    MAPK9 Polyclonal Antibody

  • Price

    245 EUR

  • Size

    50 μg

  • Catalog number

    CAC07034

Detailed information

Other name

Mitogen-activated protein kinase 9,MAP kinase 9,MAPK 9,Stress-activated protein kinase 1a,SAPK1a,Stress-activated protein kinase JNK2,c-Jun N-terminal kinase 2,MAPK9,JNK2, PRKM9,SAPK1A

Immunogen

Recombinant human Mitogen-activated protein kinase 9 protein (1-424AA)

Verified reactivity

Human, Mouse

Verified applications

ELISA,WB,IP. Recommended dilution:WB:1:1000-1:5000,IP:1:200-1:2000

Raised in

Rabbit

Clonality

Polyclonal

Protein number

P45984

Gene number

Please refer to GenBank

NCBI number

Please refer to NCBI

Purity

Caprylic Acid Ammonium Sulfate Precipitation Purified

Storage recommendation

Aliquot and store at -20°C. Minimize freezing and thawing.

Use before

1 year

Shipping requirements

Blue ice

Estimated production time

3-7 business days

Supplementary information

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock. MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.

Notes

For research use only. Not for diagnostic procedures.

Properties

If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

French translation

anticorps