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Basic information

  • Name

    Rabbit Anti-Human MAP4K4 (pThr187) Polyclonal: ATTO 488

  • Price

    397 EUR

  • Size

    100 µl

  • Catalog number

    SPC-994D-A488

Detailed information

Immunogen

A phospho-specific peptide corresponding to residues surrounding Thr187 of human MAP4K4 (AA184-190)

Antibody's target

Human MAP4K4 (pThr187)

Antibody's full description

Rabbit Anti-Human MAP4K4 (pThr187) Polyclonal

Antibody's category

Polyclonal Antibodies

Antibody's other name

Hepatocyte progenitor kinase like/germinal center kinase like kinase Antibody, HGK Antibody, HPK/GCK like kinase Antibody, KIAA0687 Antibody, M4K4_HUMAN Antibody, MAP4K4 Antibody, MAPK/ERK kinase kinase kinase 4 Antibody, MEK kinase kinase 4 Antibody, MEKKK 4 Antibody, Mitogen activated protein kinase kinase kinase kinase 4 Antibody, Nck interacting kinase Antibody, NIK Antibody

Verified applications

WB, AM

Raised in

Rabbit

Antibody's reactivity

Human

Antibody's recommended dilutions for use

WB (1:250); optimal dilutions for assays should be determined by the user.

Antibody's purified from

Peptide Affinity Purified

Recommended buffer for storage

PBS pH7.4, 50% glycerol, 0.025% Thimerosal

Antibody's concentration

See included datasheet or contact our support service

Antibody's specificity

Detects 142.101 kDa.

Storage recommendations

-20°C

Shipping recommendations

Blue Ice or 4°C

Certificate of analysis

A 1:250 dilution of SPC-994 was sufficient for detection of MAP4K4 (pThr187) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody in cell

Cytoplasm

Tissue specificity

Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.

Scientific context

MAP4K4 appears to act upstream of the JUN N-terminal pathway. It phosphorylates SMAD1 on Thr-322.

Bibliography

Refer to PubMed

Released date

29/Aug/2016

NCBI number

NP_001155032

Gene number

9448

Protein number

O95819

PubMed number

Refer to PubMed

Tested applications

To be tested

Tested reactivity

To be tested

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure link

No Data Available

Warning information

Non-hazardous

Country of production

Canada

Total weight (kg)

1.4

Net weight (g)

0.1

Stock availabilit

In Stock

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus