Rabbit Anti-Human MAP4K4 (pThr187) Polyclonal: Biotin
A phospho-specific peptide corresponding to residues surrounding Thr187 of human MAP4K4 (AA184-190)
Human MAP4K4 (pThr187)
Antibody's full description
Rabbit Anti-Human MAP4K4 (pThr187) Polyclonal
Antibody's other name
Hepatocyte progenitor kinase like/germinal center kinase like kinase Antibody, HGK Antibody, HPK/GCK like kinase Antibody, KIAA0687 Antibody, M4K4_HUMAN Antibody, MAP4K4 Antibody, MAPK/ERK kinase kinase kinase 4 Antibody, MEK kinase kinase 4 Antibody, MEKKK 4 Antibody, Mitogen activated protein kinase kinase kinase kinase 4 Antibody, Nck interacting kinase Antibody, NIK Antibody
Antibody's recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from
Peptide Affinity Purified
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 142.101 kDa.
Blue Ice or 4°C
Certificate of analysis
A 1:250 dilution of SPC-994 was sufficient for detection of MAP4K4 (pThr187) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell
Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
MAP4K4 appears to act upstream of the JUN N-terminal pathway. It phosphorylates SMAD1 on Thr-322.
Refer to PubMed
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Representative figure link
No Data Available
Country of production
Total weight (kg)
Net weight (g)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.