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Basic information

  • Name

    Mouse Anti-Human p38 alpha (MAP Kinase) Monoclonal IgG1, Clone 9F12

  • Price

    291 EUR

  • Size

    100 µg

  • Catalog number

    SMC-152D

Detailed information

Clone

9F12

Immunogen

Full length recombinant protein expressed in E.coli cells

Antibody's full description

Mouse Anti-Human p38 MAPK Monoclonal IgG1 Antibody, Clone: 9F12: Unconjugated

Antibody's category

Monoclonal Antibodies

Antibody's other name

CSAID Binding protein 1 Antibody, CSBP1 Antibody, CSBP2 Antibody, EXIP Antibody, MAP kinase MXI2 Antibody, MAPkinase p38alpha Antibody, MAPK14 Antibody, p38 ALPHA Antibody, p38 MAP kinase Antibody, p38 mitogen activated protein kinase Antibody, RK Antibody, SAPK 2A Antibody, Stress activated protein kinase 2A Antibody

Raised in

Mouse

Antibody's target

p38 MAPK

Primary research fields

Cell Signaling, Post-translational Modifications, Phosphorylation, Cancer

Brandname

None

Antibodies' applications

WB, IHC, IP, ELISA, AM

Antibody's reactivity

Human, Mouse, Rat

Antibody's dilutions

WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.

Purity

Protein G Purified

Antibody buffer for storage

PBS, 50% glycerol, 0.09% sodium azide

Antibody's concentration

1 mg/ml

Antibody's specificity

Detects ~38kDa.

Storage recommendations

-20ºC

Shipping recommendations

Blue Ice or 4ºC

Antibody certificate of analysis

Detects ~38kDa protein corresponding to p38α MAPK when loaded with 6 ng of purified p38α by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Antibody in cell

Cytoplasm , Nucleus

Tissue specificity

See included datasheet or contact our support service

Scientific context

The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).

Bibliography

1. Pearson, G. et al (2001). Endocrine Reviews 22 (2): 153-183. 2. Fan, Y. et al (2007) Mol. Cells 23 (1): 30-38. 3. Han, J. et al. (1994) Science 265: 808-811. 4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell. 115: 61-70. 6. Salojin KV, et al. (2006) J Immunol. 176 (3):1899-907. 7. Medicherla S. et al. (2006). J Pharmacol Exp Ther.318(1): 99-107.

Tested applications

To be tested

Tested reactivity

To be tested

NCBI number

NP_001306.1

Gene number

1432

Protein number

Q16539

Antibody's datasheet

Contact our support service

Representative figure link

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Representative figure legend

Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (SMC-152). Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (SMC-152) at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute. Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (SMC-152). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (SMC-152) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. Western Blot analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (SMC-152). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (SMC-152) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-p38 MAPK Antibody [9F12] used in Immunohistochemistry (IHC) on Mouse Retinal Injury Model (SMC-152) Mouse Anti-p38 MAPK Antibody [9F12] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-152) Mouse Anti-p38 MAPK Antibody [9F12] used in Western Blot (WB) on Human Cell lysates (SMC-152)

Warning information

Non-hazardous

Country of production

Canada

Total weight (kg)

1.4

Net weight (g)

0.1

Stock availability

In Stock

Description

The Mouse Anti- p38 alpha (MAP Kinase) Monoclonal IgG1, Clone 9F12 is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional isotype

IgG1

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Latin name

Mus musculus