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Basic information

  • Name

    JNK Activity Assay Kit, KinaseSTAR

  • Price

    642 EUR

  • Size

    40 assays

  • Catalog number

    K431-40

Detailed information

Kit's description

c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. The JNK Activity Immunoassay Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. Activity of the JNK is then determined in a kinase reaction using recombinant c-Jun as substrate. Phosphorylation of the c-Jun can be analyzed by Western blot analysis using a phospho-c-Jun specific antibody. The kit specifically detects JNK, other kinase activity would not be detected.

Highlights

• Detection method: Western blot analysis using a phospho:c:Jun specific antibody at 1:1000 dilutions • Species reactivity: Mammalian • Applications: The JNK Activity Immunoassay Kit utilizes a JNK:specific antibody to immunoprecipitate JNK from cell lysate. JNK:specific activity is then analyzed by determining the phosphorylation of c:Jun by Western blotting using a phospho:c:Jun specific antibody.

Kit's benefits

• Simple procedure • The kit is suitable for both research and drug discovery • The kit specifically detects JNK, other kinase activity would not be detected

Category

JNK

Kit's other name

none

Contents

• Kinase Extraction Buffer • JNK Specific Antibody • Protein A Sepharose • c-Jun Protein/ATP Mixture • Kinase Assay Buffer • Phospho-cJun Specific Antibody

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

12 months

Species reactivity

see datasheet

Applications

Inquire

Samples tested

Cell and tissue lysates

Test

Biovision supplies other types of Assays as 1.