MAP Kinase, Activated(Diphosphorylated ERK1&2) Antibody ( MAPK
MAP Kinase, ActivatedDiphosphorylated ERK1&2
Type of the antibody
IgG1 monoclonal antibody
Reacts with species:
Human, mouse, rat, yeast
A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH.
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
Keep the MAP Kinase, Activated(Diphosphorylated ERK1&2) Antibody ( MAPKat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
The MAP Kinase, Activated(Diphosphorylated ERK1&2) Antibody ( MAPK did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein(MAP) kinase pathways. The central core of each MAP kinase(MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase(MAPKKK) phosphorylates and activates a specific MAPK kinase(MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.
1. Rodriguez-Viciana, P.; Tetsu, O.; Tidyman, W. E.; Estep, A. L.; Conger, B. A.; Santa Cruz, M.; McCormick, F.; Rauen, K. A. : Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome. Science 311: 1287-1290, 2006. 2. Scholl, F. A.; Dumesic, P. A.; Barragan, D. I.; Harada, K.; Bissonauth, V.; Charron, J.; Khavari, P. A. : Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Dev. Cell 12: 615-629, 2007.
Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3
Gene Full Name
mitogen-activated protein kinase 1; mitogen-activated protein kinase 3
ERK|p38|p40|p41|ERK2|ERT1|MAPK2|PRKM1|PRKM2|P42MAPK|p41mapk|ERK1|ERT2|ERK-1|PRKM3|P44ERK1|P44MAPK|HS44KDAP|HUMKER1A|p44-ERK1|p44-MAPK|Extracellular signal-regulated kinase 2|MAP kinase isoform p42|Mitogen-activated protein kinase 2
Human(P28482), Mouse(P63085), Rat(P63086), Human(P27361), Mouse(Q63844), Rat(P21708)
Human(5594), Mouse(26413), Rat(116590), Human(5595), Mouse(26417), Rat(50689)
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.