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Basic information

  • Name

    MAP Kinase, Activated(Diphosphorylated ERK1&2) Antibody ( MAPK

  • Price

    320 EUR

  • Size

    0,1 mg

  • Catalog number

    MA1055

Detailed information

Target antigen

MAP Kinase, ActivatedDiphosphorylated ERK1&2

Clonality

Monoclonal antibody

Clone

MAPK-YT

Raised in

mice

Type of the antibody

IgG1 monoclonal antibody

Product form

freeze-dried

Reacts with species:

Human, mouse, rat, yeast

Analyses

WB,IHC-P,ICC

Immunogen

A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH.

Product configuration

Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification

Ascites

Solubilization

The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.

Storage condtions

Keep the MAP Kinase, Activated(Diphosphorylated ERK1&2) Antibody ( MAPKat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.

Tips

The MAP Kinase, Activated(Diphosphorylated ERK1&2) Antibody ( MAPK did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.

Background

In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein(MAP) kinase pathways. The central core of each MAP kinase(MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase(MAPKKK) phosphorylates and activates a specific MAPK kinase(MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.

Related articles

1. Rodriguez-Viciana, P.; Tetsu, O.; Tidyman, W. E.; Estep, A. L.; Conger, B. A.; Santa Cruz, M.; McCormick, F.; Rauen, K. A. : Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome. Science 311: 1287-1290, 2006. 2. Scholl, F. A.; Dumesic, P. A.; Barragan, D. I.; Harada, K.; Bissonauth, V.; Charron, J.; Khavari, P. A. : Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Dev. Cell 12: 615-629, 2007.

Gene Name

MAPK1; MAPK3

Protein Name

Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3

Gene Full Name

mitogen-activated protein kinase 1; mitogen-activated protein kinase 3

Synonyms

ERK|p38|p40|p41|ERK2|ERT1|MAPK2|PRKM1|PRKM2|P42MAPK|p41mapk|ERK1|ERT2|ERK-1|PRKM3|P44ERK1|P44MAPK|HS44KDAP|HUMKER1A|p44-ERK1|p44-MAPK|Extracellular signal-regulated kinase 2|MAP kinase isoform p42|Mitogen-activated protein kinase 2

Uniprot ID

Human(P28482), Mouse(P63085), Rat(P63086), Human(P27361), Mouse(Q63844), Rat(P21708)

Entrez GeneID

Human(5594), Mouse(26413), Rat(116590), Human(5595), Mouse(26417), Rat(50689)

Properties

If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

French translation

anticorps