Need help?

+390236006593Hours: 8am-7pm M-Fri

Basic information

  • Name

    MAPK1/3 Antibody

  • Price

    320 EUR

  • Size

    0,1 mg

  • Catalog number

    PA1049

Detailed information

Target antigen

MAPK1/3

Clonality

Polyclonal antibody

Clone

Polyclonal antibody

Raised in

rabbit

Type of the antibody

IgG polyclonal antibody

Product form

freeze-dried

Reacts with species:

human, rat Predicted to work with: mouse

Analyses

WB,IHC-P

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MAPK1/3(171-184aa ARVADPDHDHTGFL), identical to the related rat and mouse sequences.

Product configuration

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification

Immunogen affinity purified.

Solubilization

The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml

Storage condtions

Keep the MAPK1/3 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.

Tips

The MAPK1/3 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.

Background

MAPK1(ERK2) shares high homology with MAPK3(ERK1). MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Mitogen-activated protein(MAP) kinases [also known as Erks] have been established to function as important mediators of signal transduction by growth factor receptors. ERK1/ERK2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level. ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF at amino acids 117 and 201 within HMG boxes 1 and 2, preventing their interaction with DNA. Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2.

Related articles

1. Bhalla, U. S.; Ram, P. T.; Iyengar, R. : MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Science 297: 1018-1023, 2002. 2. Li, L.; Wysk, M.; Gonzalez, F. A.; Davis, R. J. : Genomic loci of human mitogen-activated protein kinases. Oncogene 9: 647-649, 1994.

Gene Name

MAPK1

Protein Name

Mitogen-activated protein kinase 1(MAP kinase 1/MAPK 1)

Gene Full Name

mitogen-activated protein kinase 1

Synonyms

ERT1|Extracellular signal-regulated kinase 2

Uniprot ID

P28482

Properties

If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

French translation

anticorps