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Basic information

  • Name

    MAP3K4/MEK kinase-4 Antibody, Cy3 Conjugated

  • Price

    350 EUR

  • Size

    0.1ml

  • Catalog number

    bs-11883R-Cy3

Detailed information

Long name

MAP3K4/MEK kinase-4 Polyclonal Antibody, Cy3 Conjugated

Category

Conjugated Primary Antibodies

Conjugation

Cy3

Host Organism

Rabbit (Oryctolagus cuniculus)

Target Antigen

MAP3K4/MEK kinase-4

Specificity

This is a highly specific antibody against MAP3K4/MEK kinase-4.

Modification

Unmodified

Modification site

None

Clonality

Polyclonal

Clone

Polyclonal antibody

Concentration

1ug per 1ul

Source

KLH conjugated synthetic peptide derived from human MAP3K4/MEK kinase-4

Tested applications

IF(IHC-P)

Recommended dilutions

IF(IHC-P)(1:50-200)

Crossreactivity

Human, Mouse, Rat

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Antigen background

Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK path-way. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.

Purification method

This antibody was purified via Protein A.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Excitation Emission

512,550nm/570,615nm

Synonyms

FLJ42439; KIAA0213; M3K4_HUMAN; MAP three kinase 1; MAP3K4; MAPK/ERK kinase kinase 4; MAPKKK4 antibodyMEK kinase 4; MEKK 4; MEKK4; Mitogen-activated protein kinase kinase kinase 4; MTK1; PRO0412; RP3-473J16.4

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-MAP3K4/MEK kinase-4 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.

Additional conjugation

cy3 conjugation kit

French translation

anticorps