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Basic information

  • Name

    Anti-MAP3K4/MEK kinase-4 (Polyclonal), ALEXA Fluor 594

  • Price

    489 EUR

  • Size

    100 microliters

  • Catalog number

    GENTObs-11883R-A594

Detailed information

Type

Conjugated Primary Antibody

Conjugated with

ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Target Protein/Peptide

MAP3K4/MEK kinase-4

Specificity

This antibody reacts specifically with MAP3K4/MEK kinase-4

Modification

No modification has been applied to this antibody

Modification site

None

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Concentration

1ug per 1ul

Subcellular locations

N/A

Antigen Source

KLH conjugated synthetic peptide derived from human MAP3K4/MEK kinase-4

Gene ID

N/A

Swiss Prot

N/A

Applications

IF(IHC-P)

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Background information

Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK path-way. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.

Purification method

Purified by Protein A.

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Excitation emission

590nm/617nm

Synonyms

FLJ42439; KIAA0213; M3K4_HUMAN; MAP three kinase 1; MAP3K4; MAPK/ERK kinase kinase 4; MAPKKK4 antibodyMEK kinase 4; MEKK 4; MEKK4; Mitogen-activated protein kinase kinase kinase 4; MTK1; PRO0412; RP3-473J16.4

Also known as

MAP3K4/MEK kinase-4 Polyclonal Antibody

Other name

Anti-MAP3K4/MEK kinase-4 Polyclonal

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Properties

For facs or microscopy Alexa 1 conjugate.

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.