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Basic information

  • Name

    Anti-MAPKAP Kinase 2 (Polyclonal), ALEXA Fluor 594

  • Price

    489 EUR

  • Size

    100 microliters

  • Catalog number

    GENTObs-2908R-A594

Detailed information

Type

Conjugated Primary Antibody

Conjugated with

ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Target Protein/Peptide

MAPKAP Kinase 2

Specificity

This antibody reacts specifically with MAPKAP Kinase 2

Modification

No modification has been applied to this antibody

Modification site

None

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Concentration

1ug per 1ul

Subcellular locations

Cytoplasm, Nucleus

Antigen Source

KLH conjugated synthetic peptide derived from human MAPKAP Kinase 2

Gene ID

9261

Swiss Prot

P49137

Applications

IF(IHC-P)

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus), Bovine

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Background information

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA, HSF1, HSP27/HSPB1, KRT18, KRT2, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA, PABPC1 and TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA and PARN, leading to stabilize GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.

Purification method

Purified by Protein A.

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Excitation emission

590nm/617nm

Synonyms

MK2; MK-2; MAPKAP-K2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAPK-2; MAPKAPK2

Also known as

MAPKAP Kinase 2 Polyclonal Antibody

Other name

Anti-MAPKAP Kinase 2 Polyclonal

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Properties

For facs or microscopy Alexa 1 conjugate.

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.